TurboFectin 8.0 Transfection Reagent (1 mL in 1 vial) from OriGene Technologies

Product Specs
ItemTurboFectin 8.0 Transfection Reagent (1 mL in 1 vial)
CompanyOriGene Technologies
Price Pricing Info Supplier Page View Company Product Page
Catalog NumberTF81001
Quantity1 ml in 1 vial
TypeTransfection Reagents
ApplicationsTransfection

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OriGene Technologies

9620 Medical Center Drive # 200

Rockville, Maryland 20850

United States

Phone: 1-888-267-4436 (U.S. only) / 301-340-3188
301-340-9254

Company Profile

Website: http://www.origene.com

(1) Pooled endogenous protein tagging and recruitment for scalable discovery of effectors for induced proximity therapeuticsResearch SquareOctober 18, 2023Yevgeniy V. Serebrenik, Yevgeniy Serebrenik, Timothé Maujean, George M. Maujean, George Burslemcm dish in 15 ml of antibiotic-free media. Transfection was performed with TurboFectin 8.0 (OriGene, TF81001). 85 μl reagent (3 μl reagent : 1 μg DNA) was incubated in 1.25 ml Opti-MEM, and a separate solution

(2) ONECUT2 Activates Diverse Resistance Drivers of Androgen Receptor-Independent Heterogeneity in Prostate CancerbioRxivOctober 18, 2023Chen Qian, Qian Yang, Mirja Rotinen, Rongrong Huang, Hyoyoung Kim, Brad Gallent, Yiwu Yan, Radu M. Cadaneanu, Baohui Zhang, Salma Kaochar, et al.luciferase backbone construct, and the pRL-SV40 vector (Promega #E2231) with Turbofectin 8.0 (Origene, #TF81001). After transfection overnight, cells were washed and treated for 6 hours with CSRM-617 (10uM). Luciferase

(3) The Axin scaffold protects the kinase GSK3β from cross-pathway inhibitionElifeAugust 7, 2023Maire Gavagan, Noel Jameson, Jesse G Zalatanwells were transfected with 50 μL Opti-MEM media containing 1 μg DNA and 1.5 μL TurboFectin (Origene TF81001). Twenty-four hr following transfection, cells were starved by replacing media with 1% FBS DMEM. Twenty-four

(4) TMEM106B is a receptor mediating ACE2-independent SARS-CoV-2 cell entryCellAugust 3, 2023Jim Baggen, Maarten Jacquemyn, Leentje Persoons, Els Vanstreels, Valerie E. Pye, Antoni G. Wrobel, Valeria Calvaresi, Stephen R. Martin, Chloë Roustan, Nora B. Cronin, et al.plasmids encoding spike/mNeonGreen, TMPRSS2, and Luc, ACE2 or TMEM106B using Turbofectin 8.0 (OriGene TF81001) according to the manufacturer’s protocol. Cells were scanned at regular time intervals in an Incucyte

(5) Impaired mitochondrial dynamics and removal of the damaged mitochondria in diabetic retinopathyFront Endocrinol (Lausanne)June 21, 2023Kumari Alka, Jay Kumar, Renu A. Kowluruwere transfected with Mfn2 overexpressing plasmids using Turbofectin reagent (Cat. No. RG202218 and TF81001; OriGene, Rockville, USA), as described previously (13). After incubating for eight hours with the plasmids,

(6) Cationic amphiphilic antihistamines inhibit STAT3 via Ca2+-dependent lysosomal H+ effluxCell ReportsFebruary 17, 2023Bin Liu, Ran Chen, Yidan Zhang, Jinrong Huang, Yonglun Luo, Susanne Rosthøj, Chenyang Zhao, Marja Jäättelänot otherwise stated, plasmid transfections were performed by using TurboFectin 8.0 (Origene, CAT#: TF81001) or Lipofectamine 3000 transfection agents (Thermo Fisher Scientific, Cat# L3000008) according to the

(7) Targeting the secreted RGDKGE collagen fragment reduces PD‑L1 by a proteasome‑dependent mechanism and inhibits tumor growthOncology reportsFebruary 1, 2023Caron JM, Han X, Lary CW, Sathyanarayana P, Remick SC, Ernstoff MS, Herlyn M, Brooks PC.plates) were transfected to Β16F10 cells at 37°C with TurboFectin™ 8.0 transfection reagent (cat. no. TF81001) from OriGene Technologies, Inc. according to the manufacturer's instructions. After 24 h of transfection,

(8) In vivo induction of activin A-producing alveolar macrophages supports the progression of lung cell carcinomaNature communicationsJanuary 17, 2023Taniguchi S, Matsui T, Kimura K, Funaki S, Miyamoto Y, Uchida Y, Sudo T, Kikuta J, Hara T, Motooka D, et al.shRNA (TR30012, Origene) were transfected into Plat-E cells with TurboFectin 8.0 Transfection Reagent (TF81001, Origene). Two days after transfection, AMJ2-C11 cells were incubated with retroviral supernatant from

(9) Loss of the Volume-regulated Anion Channel Components LRRC8A and LRRC8D Limits Platinum Drug EfficacyCancer Research CommunicationsDecember 5, 2022Carmen A. Widmer, Ismar Klebic, Natalya Domanitskaya, Morgane Decollogny, Denise Howald, Myriam Siffert, Paul Essers, Zuzanna Nowicka, Nadine Stokar-Regenscheit, Marieke van de Ven, et al.pOZ-Lrrc8d-Myc or empty pOZ-Myc using Turbofectin transfection reagent (Origene, LabForce catalog no. TF81001). The next day, virus‐containing supernatant was collected, filtered through a 0.45 μm filter before

(10) Receptor-interacting protein kinase 2 (RIPK2) stabilizes c-Myc and is a therapeutic target in prostate cancer metastasisNature CommunicationsDecember 1, 2022Yiwu Yan et al.confluency of ~50%, a specified amount of plasmid was transfected into cells using Turbofectin 8.0 (Origene #TF81001) by following the manufacturer’s instructions. In the RIPK2 and c-Myc co-transfection experiments, 0.5

(11) CRISPR-mediated multiplexed live cell imaging of nonrepetitive genomic loci with one guide RNA per locusNature CommunicationsDecember 1, 2022Patricia A. Clow, Menghan Du, Nathaniel Jillette, Aziz Taghbalout, Jacqueline J. Zhu, Albert W. ChengsgRNA-15xPBS plasmid and 40 ng of Clover-PUFc fusion plasmid DNA using 3.5 μl Turbofectin (OriGene #TF81001). Media was changed at 24 h post-transfection. HCT116/RAD21-mAID/dCas9 cells were seeded at density

(12) A cohesin traffic pattern genetically linked to gene regulationNature structural & molecular biologyDecember 1, 2022Valton AL, Venev SV, Mair B, Khokhar ES, Tong AHY, Usaj M, Chan K, Pai AA, Moffat J, Dekker J.chloroform extraction and ethanol precipitation. HAP1 cells were transfected using turbofectin (Origene, TF81001) following the manufacturer’s recommendations. The differences between the different construct transfections

(13) miR-637 Prevents Glioblastoma Progression by Interrupting ZEB2/WNT/β-catenin CascadesCellular and Molecular NeurobiologyOctober 1, 2022Wei Wang, Zilong Zhao, Shuai Han, Di WuZEB2 (GFP-sh-ZEB2, #TG308288), pGFP-V-RS vector (#TR30007), and Turbofectin 8.0 transfection reagent (#TF81001) were purchased from OriGene (Rockville, MD, USA). Cells were transfected with 1 μg GFP-sh-ZEB2 or pGFP-V-RS

(14) AKT constitutes a signal-promoted alternative exon-junction complex that regulates nonsense-mediated mRNA decayMolecular cellAugust 4, 2022Cho H, Abshire ET, Popp MW, Pröschel C, Schwartz JL, Yeo GW, Maquat LE."Trypsin-EDTA (0.25%), phenol red",Gibco,25200056 ACCUTASE™ Cell detachment solution,Stem Cell Technologies,07920 Turbofectin 8.0,Origene,TF81001 Lipofectamine™ 2000,Invitrogen,11668019 Lipofectamine™ RNAiMAX,Invitrogen,13778075 Opti-MEM™ I Reduced Serum Medium

(15) Loss of function of renal Glut2 reverses hyperglycaemia and normalises body weight in mouse models of diabetes and obesityDiabetologiaJune 1, 2022Leticia Maria de Souza Cordeiro, Lauren Bainbridge, Nagavardhini Devisetty, David H. McDougal, Dorien J. M. Peters, Kavaljit H. Chhabraor Glut2-expressing plasmid (MG208388; OriGene Technologies) using TurboFectin transfection reagent (TF81001; OriGene Technologies) according to the manufacturer’s protocol. Three days after the transfection,

(16) LncRNA HOTAIR sponges miR-301a-3p to promote glioblastoma proliferation and invasion through upregulating FOSL1Cellular SignallingJune 1, 2022Shanchun Guo, Pendelton King, Emily Liang, Alyssa A. Guo, Mingli Liupurchased from Calbiochem (La Jolla, CA). TurboFectin was purchased from OriGene (Rockville, MD, cat. no. TF81001). 2.2. Plasmid, siRNA, and miRNAs Control scrambled siRNA (cat. no. D-001810–01-05) and ON-TARGETplus

(17) Regulated IRE1α-dependent decay (RIDD)-mediated reprograming of lipid metabolism in cancerNature communicationsMay 6, 2022Almanza A, Mnich K, Blomme A, Robinson CM, Rodriguez-Blanco G, Kierszniowska S, McGrath EP, Le Gallo M, Pilalis E, Swinnen JV, et al.in combination with the donor DNA (OriGene, #KN201959-D). TurboFectin transfection reagent (OriGene #TF81001) was used according to the manufacturer’s instructions with a 1:2 ratio of DNA to transfection reagent.

(18) Unbiased proteomic profiling reveals the IP3R modulator AHCYL1/IRBIT as a novel interactor of microtubule-associated protein tauJournal of Biological ChemistryApril 1, 2022Lena Wischhof, Aasha Adhikari, Mrityunjoy Mondal, Anaïs Marsal-Cots, Jacek Biernat, Eva Maria Mandelkow, Eckhard Mandelkow, Dan Ehninger, Pierluigi Nicotera, Daniele Banoplating. HeLa cells were transfected with HA-YFP or HA-tau40 using TurboFectin 8.0 (Origene; catalog no.: TF81001) according to the supplied protocol. After 24 h, cells were retransfected with scramble (Ambion; catalog

(19) KDM6B interacts with TFDP1 to activate P53 signalling in regulating mouse palatogenesiseLifeFebruary 25, 2022Tingwei Guo, Xia Han, Jinzhi He, Jifan Feng, Junjun Jing, Eva JaneÄkovÃ¡, Jie Lei, Thach-Vu Ho, Jian Xu, Yang Chai(OriGene) transfection was performed following the manufacturer’s protocol (QIAGEN, 301704, and OriGene, TF81001). Briefly, siRNA was transfected into cells in 24-well plates at 10 nM for 3 days followed by qPCR and

(20) The KRAB Domain of ZNF10 Guides the Identification of Specific Amino Acids That Transform the Ancestral KRAB-A-Related Domain Present in Human PRDM9 into a Canonical Modern KRAB-A DomainInternational journal of molecular sciencesJanuary 19, 2022Lorenz P, Steinbeck F, Krause L, Thiesen HJ.(E2311, Promega GmbH, Walldorf, Germany), whereas HAP1 cell lines were transfected with Turbofectin 8.0 (TF81001, OriGene Technologies GmbH, Herford, Germany) according to the manufacturer’s recommendations at 3 µL

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